Composite

Part:BBa_K2486177:Design

Designed by: Guilherme Meira Lima   Group: iGEM17_USP-Brazil   (2017-11-01)


Synthetic Toehold Switch RNA with Lac Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 942


Design Notes

The design of this part was inspired by the synthetic toehold switch regulated by a T7 promoter. Since we intended to use this part in another chassis, we decided create this part along with a Lac promoter.


Source

Adapted from: Green, A., Silver, P., Collins, J., & Yin, P. (n.d.). Toehold Switches: De-Novo-Designed Regulators of Gene Expression. Cell, 925-939.

References